A micromethod for estimating osmotic fragility of erythrocytes

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Graphic analysis of osmotic fragility of erythrocytes.

A precise and highly reproducible method for analyzing the osmotic fragility of erythrocytes with a minute amount of blood (less than 10 mul) is described. The osmotic fragility curves are recorded with a coil planet centrifuge with accessories and a scanning photodensitometer. The recorded curves are transcribed by a DuPont Curve Resolver and their components are analyzed. Normal fragility cur...

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Effect of parathyroid hormone on osmotic fragility of human erythrocytes.

The survival of erythrocytes (RBC) is shortened in uremia, and it has been shown that calcium influx into RBC evoked crenation and increased their rigidity. The high blood levels of parathyroid hormone (PTH) may augment entry of calcium into RBC and hence affect their integrity. We examined the effect of PTH on osmotic fragility of human RBC and investigated the mechanisms through which PTH int...

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Osmotic Fragility of Human Erythrocytes is Altered by Porphyrin Compounds

The chemical specificity of Porphyrin derivatives, Tetraphenylporphinesulfonate (TPPS), 5, 10, 15, 20-Tetrakis (4-sulfonatophenyl) porphyrinato Iron (III) Chloride (FeTPPS) and 5, 10, 15, 20-Tetrakis (4-sulfonatophenyl) porphyrinato Iron (III) nitrosyl Chloride (FeNOTPPS), on osmotic fragility of erythrocytes was examined in vitro. TPPS increased the osmotic fragility of erythrocytes more effec...

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Osmotic fragility of erythrocytes in Duchenne muscular dystrophy.

Osmotic fragility of erythrocytes was studied in ten Duchenne dystrophic patients and eight healthy controls. Lysis started in Duchenne crythrocytes at a concentration of 6.5 g/l NaC1 and in the control erythrocytes at a concentration of 5.5 gIl NaCI. At all concentrations of sodium chloride (from 6.5 g/l to 4.5 g/l) significantly greater lysis was observed in the erythrocytes of dystrophic pat...

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III RESULTS OF TESTS FOR HAEMOLYSIS AND FOR OSMOTIC FRAGILITY OF RED BLOOD CELLS Osmotic Fragility

SYNOPSIS Nineteen patients with morphological abnormalities of the red blood cells are described, and these formed approximately 3 % of the total cases of cardiac valvular disease. In two patients the abnormal blood film developed after the insertion of an aortic and mitral valve prosthesis respectively, but in another two patients the abnormal blood film was corrected by aortic valve surgery. ...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1964

ISSN: 0021-9746

DOI: 10.1136/jcp.17.1.96